Impure reagents can lead to downstream design flaws and finished product instability, so it is crucial we ensure reagents are free of contaminants. Mologic has invested heavily in both staff and equipment capability to fully assess reagent purity. To do this, we utilize chromatography tools (ex. FPLC, HPLC), electrophoresis tools (ex. SDS-PAGE/ Western blot) and spectrometry tools (ex. UV/VIS, fluorimetry, dynamic light scattering, and mass spectrometry).
The characterization of biomolecule activity is paramount to its utility and allows for appropriate downstream inclusion. Our capability uses information gathered from peptide epitope mapping, bioactivity testing, flow cytometry / imaging microscopy and label free kinetic analysis (which is superior to SPR results) to optimize the reagent’s ability to exceptionally function in the finished product.
Our development packages (immunoassay, antibody, recombinant protein) include a range of analytical testing methods to ensure quality and activity of critical reagents specifically relating to identity, purity, and stability. We are also able to offer reagent analysis as a standalone service to provide characterization for already developed materials.