TIMP-2 Lateral Flow Kit
For Research Use Only
B.I.T.S. TIMP-2 Lateral Flow Kit
Lateral Flow assay
Urine & Serum
Intra-assay CV% < 5.4
Inter-assay CV % < 8.0
Average 111.1% Range 104 – 123.8%
Average 108.1% Range 85.9 – 120.4%
1 in 5, 1 in 10, 1 in 20 with recovery: Urine – <85.9% Serum <112.1%
1 in 200K, 1 in 400K, 1 in 800K, with recovery < 103.2%
Tissue inhibitor of metalloproteinase-2 (TIMP2) from elastin.
The unique B.I.T.S.® TIMP-2 Lateral Flow Kit has been developed by Mologic to provide fast, accurate assays of TIMP-2 in urine and serum. It’s unique because it has been tested against TIMP-1,, IL-1 beta, IL-4, IL-6, IL-8, IL-10, TNF alpha, MMP-8 and MMP-9 for cross-reactivity. The B.I.T.S.® assay needs no sample work-up.
The simple procedure makes it compatible with any research laboratory with basic lateral flow visualisation.
Refined and simplified step-wise procedure minimises operator error and significantly reduces time between sampling and results. Time saved in executing the assay will give you more time to do more research.
Kits manufactured to the highest quality by Mologic scientists who have developed these assays from scratch to ensure stability and batch to batch reproducibility are better than industry standards. Despite this commitment to quality, the price of the kits is in line with the competition – quality without compromise.
Mologic has invested time and resources over the past 5 years into the task of producing anti-TIMP-2 antibodies of the highest possible quality, with tailor-made specificity and sensitivity to ensure that the assay gives the right sensitivity and specificity for use in research on metastatic and non-metastatic malignancies as well as chronic inflammatory diseases such as rheumatoid arthritis and periodontitis .
The structure and function of the extracellular matrix (ECM) is vital in a number of physiological processes including embryonic development, tissue repair and remodelling . ECM structure is ultimately regulated by the balance of extracellular proteases and protease inhibitors. Imbalance of this is implicated in rheumatoid arthritis, tumour cell invasion and metastasis .
The structure and function of the extracellular matrix (ECM) is vital in a number of physiological processes including embryonic development, tissue repair and remodelling . ECM structure is ultimately regulated by the balance of extracellular proteases and protease inhibitors. Differences in specificity and expression
profiles of these proteases and their inhibitors are key in the strict structural regulation of the ECM. Imbalance of these is implicated in rheumatoid arthritis, tumour cell invasion and metastasis .
The family of proteins called tissue inhibitors of metalloproteinase (TIMPs) are endogenous inhibitors of matrix metalloproteinase (MMP) function. To date four isoforms have been identified. One member of this family is TIMP-2, a non-glycosylated, 22KDa protein. While other TIMP members are inducible, TIMP-2 is expressed constitutively and inhibits the activity of all MMPs .
Serum levels of TIMP-2 in healthy individuals are typically 54 to 141ng/ml .
1. Equilibrate all materials and prepared reagents to room temperature (18-25˚C) prior to use.
2. Remove the lateral flow device from its pouch and place it on a flat surface and test within 10 minutes of opening.
3. Remove device from the foil pouch just prior to use and use within 10 minutes.
4. Add 80μL of diluted sample to the round well at the base of the device using a pipette.
5. Read the test line result against the score card exactly 10 minutes after adding the sample to the device.
6. Any trace of a pink line in the test area indicates a positive result. Any results read outside 10 minutes should be considered invalid and must be repeated.
Determine the unknown sample concentration from the score card and multiply the value by the dilution factor 5 (for urine) or 50 (for serum).
TYPICAL STANDARD CURVE
Data provided for demonstration purposes only. A typical standard curve is shown below with the standards starting at 200ng/mL. The standard curve can be used to determine unknown levels of TIMP-2 in urine samples.
The lowest level of detection (LLOD) of TIMP-2 is typically 0.19ng/mL. The LLOD was determined by adding three standard deviations from the mean reader value of twenty zero standard replicates and calculating the corresponding concentration.
. Frantz C et al. The extracellular matrix at a glance.
Journal of Cell Science (2010). 123, 4195-4200
. Löffeck S et al. Biological role of matrix metalloproteinases: a critical balance.
European Respiratory Journal (2011). 38, 191–208
. Brew K et al. The tissue inhibitors of metalloproteinases (TIMPs): An ancient family with structural and functional diversity.
Biochimica et Biophysica Acta (2010). 1803, 55–71.
. Gómez D et al. Tissue inhibitors of metalloproteinases: structure, regulation and biological functions.
European Journal of Cell Biology (1997). 74, 111–122.
. Groblewska M et al. Serum levels and tissue expression of matrix metalloproteinase 2 (MMP-2) and tissue inhibitor of metalloproteinases 2 (TIMP-2) in colorectal cancer patients.
Tumour Biology (2014). 35, 3793–3802.
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